Health Status as a Risk Factor:

A Systems and Unit Perspective

Cate Dewey and Bob Friendship

Department of Population Medicine, 50 Stone Road E., University of Guelph, Guelph, ON, Canada, N1G 2W1

Introduction

Disease is most important when a naive herd becomes positive. Then the cost of disease is dramatic and the need for disease prevention is obvious. But it is too late, the piglets die, the sows abort, the growers cough, and the finishers stop growing. If your herd has every pig disease it is time to repopulate, if not, it is time to formulate a disease prevention strategy. This paper will outline disease transmission and prevention strategies.

Adequate Contact

Why do pigs get sick? The number of sick animals on a farm at a specific time is determined by the number of susceptible animals, the probability of an effective contact, and the incubation period of the disease (Reed-Frost Model). In a naive herd we can assume we have susceptible animals. In other herds, susceptible animals are those without immunity to disease. Adequate contact is the number of E coli organisms required to cause diarrhea. For example, if a baby pig was exposed to one E coli bacterium, it probably would not get diarrhea. The number of organisms required depends on the disease, the age of the pig, and the amount of stress in the pig's environment. A pig in a cold room will need fewer E coli bacteria than a pig in a warm room. The incubation period is the time between exposure to the organism to the time when the first clinical signs are evident. Pigs infected with TGE will shed the virus in large numbers for two weeks and then the volume of viruses shed decreases over time. TGE has an incubation period of 18 hours, but other diseases such as APP (A. pleuropneumonia) and enzootic pneumonia will not spread to all naive animals as quickly.

Cost of Disease

A sow unit that breaks with TGE can expect to lose four to six weeks of newborn pigs and have a reduced farrowing rate for six weeks. A similar herd that breaks with PRRS loses half of the weaned pigs for two months and one-quarter of the weaned pigs for a further two months and experiences ongoing morbidity and mortality in the nursery.

Disease Entry

A knowledge of all potential entry routes for disease agents is an important prelude to developing a comprehensive security program. General considerations include:

The chance that a herd will break with a disease is dependent on the probability of bringing enough bacteria or viruses (disease organisms) into the barn to provide an adequate contact for the susceptible pigs.

Pig-To-Pig Spread of Disease

By far, the greatest risk of disease entry occurs from the introduction of pigs onto the farm. There are two important principles involved in preventing disease entry when newly purchased breeding-stock are brought into the herd. Firstly, it is important to know the health status and biosecurity program of the herd selling the gilts and boars.

Breeding-stock supplier herds should be inspected by veterinarians to assess the herd for clinical evidence of disease and rate the biosecurity of the farm by awarding points for such measures as location away from other pig farms, locked doors, no introduction of live animals, and precautions taken to wash and disinfect trucks. The higher the biosecurity score, the more certain the purchaser is that the disease status of the supplier herd will remain the same. In addition, breeding-stock herds can be tested for the presence of diseases using serology, bacterial cultures, and slaughter check evaluations.

Table 1. Diseases found in various herd types.
Herds derive by cesarian have

Diseases clean herds acquire over time

Diseases kept out of clean herds
E coli Enzootic pneumonia APP
Erysipelas Glassers Mange
Illeitis Rhinitis
Parvovirus Swine dysentery

Pigs provide the best opportunity for the introduction of disease. Bacteria and viruses that cause pig diseases multiply very well in the pig. Because of this, pigs with clinical disease shed large volumes of disease organisms. Pigs that carry disease organisms, (perhaps on their tonsils) tend to shed large numbers of disease organisms after they have been stressed. A good example of stress is the transportation of breeding stock animals.

Herds that maintain a "closed" herd policy so that new genetic material is introduced only by artificial insemination, cesarian-derived piglets, or embryo transfer greatly reduce the risk of introducing disease, but certain diseases can still be spread using these techniques. In all likelihood, embryo transfer is the safest, but the most impractical of the three techniques. Diseases that can be transmitted in semen include pseudorabies, PRRS, parvovirus, and leptospirosis.

Methods used to reduce the pig-to-pig transmission of diseases include maintaining a closed herd, purchasing pigs from only one source, and isolation and testing of breeding stock prior to introduction to your herd (Tables 2, 3). The cost of a closed herd is reduced genetic diversity which may mean lower productivity as measured by reproductive parameters, weight gain, feed to gain, and/or back fat. However, closed herds can maintain freedom from diseases that are spread by pig-to-pig contact. Purchasing pigs from multiple sources increases the chance of disease introduction exponentially. If the chance of disease introduction from one source is 5%, the chance from two sources is 25%.

Even purchasing from one source can be a risk because the herd may have recently broken with a new disease. To decrease the chance of disease introduction, reduce the frequency of gilt purchases. To do this you will have to buy gilts of various ages and have sufficient room to house the gilts in your own facility.

Table 2. Diseases prevented or clinical signs reduced by various management factors.
Factor Disease and/or Agent Possible
All-in / All-out Clostridium

Enzootic pneuomonia

Salmonella

Rotavirus

PRRS

Artificial Insemination PRRS (-ve)
Batch farrow Chronic TGE PRRS
Breeding stock testing Leptospirosis (type)

Pasteurella type D

Salmonella

APP

PRRS

Glassers

Depopulate & C-section Most diseases Illeitis
Isolation unit & testing TGE PRRS
Medicated Early Weaning Atrophic rhinitis

M hyopneumonia

Illeitis ?

Strep suis ?

Segregated Early Weaning Swine dysentery

Atrophic rhinitis

APP PRRS ?
Single source Strep. suis
Vaccines Atrophic rhinitis

Erysipelas

Leptospirosis

Enzootic pneumonia

E coli

Glassers

Parvo

PRRS?



To decrease the chance of introducing disease, set up an isolation unit. How long you keep the gilts in isolation will depend on which disease you are trying to prevent and whether or not you wish to include an acclimatization period for the gilts. Each disease has a specific incubation period (the time from exposure to the disease to the onset of clinical signs) and a time from exposure to a measurable immune response (Table 3 ).

PRRS has its own set of diagnostic problems. Even if gilts come from a PRRS positive herd, they may be negative on serological tests at six months of age. By transporting gilts, we expect some will shed the virus and eventually test positive serologically or at least transmit the virus to susceptible nursery age pigs. Gilts must be kept in isolation units for 60 days if we are to ensure that they will not bring PRRS into a unit. This is because PRRS antibodies do not last a long time in positive animals and PRRS may not be transmitted easily from the gilts to the naive nursery pigs.

Table 3. Expected time of occurrence of clinical signs and antibodies to appear in weaned pigs exposed to gilts carrying disease agents.
Organism/ Disease Clinical Signs Antibodies
A. pleuropneumonia 3 weeks 30 days
Atrophic rhinitis 6 weeks 30 days
Enzootic pneumonia 2 weeks 28 days
Leptospirosis unknown 30 days
PRRS unknown 30 days
Strep suis 4 weeks 40 days
Swine Dysentery 4 weeks 30 days
TGE 1 week 14 days


Procedures for the Use of an Isolation Unit

Table 4. Appropriate age and sample sizes required for serological testing.
Agent Age a prevb / nc
APP (serotypes) 3-5 mos 8% / 33

30% / 8

Leptospira (serovar) sows 10% / 28
Enzootic pneumonia growers 10% / 28
PRRS virus 8-10 weeks 80% / 6

40% / 11 20% / 23

Pseudorabies sows 10% / 28
TGE 3-6 weeks 20% / 13

a age group with best chance of positive test
b minimum prevalence of positive animals
c number of animals required to detect at least one test positive animal

Isolation time provides a good opportunity to prepare the gilts for the breeding herd with vaccination, boar exposure for the onset of puberty, and perhaps breeding with an infertile boar.

The length of time required for isolation is dependent on the incubation time of the disease and the time it takes for a measurable immune response. The animals are tested on arrival at the isolation facility, exposed to naive herd animals after 30 days and retested prior to moving into the herd at 60 days.

Secondary Sources of Infection

The second best opportunity to transmit disease is via secretions from pigs such as feces, semen, and mucus. Certain diseases such as PRRS are transmitted very well in semen. To prevent PRRS from entering a herd, the semen used for AI must be certified free of virus. Manure is a very good vehicle for disease transmission. Most viruses and bacteria will live for extended periods of time if kept moist in manure or mucus, particularly if it is kept cold. For example, TGE is killed rapidly at room temperature but can survive for 6 months at -20oC. When stored at +21oC for 4 days there was sufficient virus in the manure to cause TGE in susceptible pigs, but by 10 days the virus particles had all died.

Dead stock removal is very important for a number of reasons. Keep rendering trucks far from the swine facility as they can harbour large numbers of disease organisms - even those that are not currently in your facility. If you dispose of dead stock on your own property these pigs can be a source of ongoing contamination. This is particularly true for diseases that can live in rodents, dogs and cats.

Table 5. Diseases transmitted by non-pig methods.
Factor Disease and/or Agent Possible
Air

Distance from other barns

M hyopneumonia

M. hyosynoviae

P multocida

APP

Influenza

Strep suis

PRRS ?
Birds PRRS TGE
Coveralls and boots

(manure, mucus, and dust)

Clostridium

Rotavirus

Swine dysentery

PRRS

TGE

APP

Dogs and cats Erysipelas

Swine dysentery

TGE Strep suis ?
Feed Bins Salmonella TGE
Foot Baths Salmonella TGE
Isolation unit PRRS (60 days)

APP (42 days)

People --- --- ---
Rodents Erysipelas

Leptospirosis

Salmonella

Swine dysentery

EMC

Illeitis

TGE

Shower Swine dysentery TGE

Rotavirus

APP ?

Strep suis ?

Time SIV

Glassers

Truck

Clean & disinfected

PRRS

Swine dysentery

APP

TGE



Birds, Rodents, and Other Animals

Birds can transmit avian tuberculosis, TGE, and erysipelas to pigs. PRRS virus which has been shown to be shed experimentally by ducks may be spread from farm-to-farm via birds. Birds have been incriminated in the spread of foot and mouth disease and salmonella, but this has seldom been proven.

Serpulina hyodysenteriae, the causal organism of swine dysentery, is carried by mice for months. Rats and mice carry and shed Salmonella typhimurium and Leptospira icterohemorrhagiae. Various animals can carry Lawsonia intracellularis, the cause of porcine proliferative enteropathy (ileitis).

Trucks, Boots, and Equipment

Vehicles hauling pigs spread disease through transfer of manure and aerosolized organisms. Many swine disease organisms survive for extended periods of time in manure and urine. The following is a partial list of diseases that survive well in the environment under certain conditions:

Table 6. Survival of organisms on inanimate objects.
Organism Survival
TGE virus Sensitive to sunlight and warm temperatures. Will survive for only 6 hours in sunlight, for about 1 week at 20oC, and indefinitely if frozen (> 1 year)
Pseudorabies virus Can survive for 14 days in urine, 7 days in well water, and about 2 days in manure
Parvovirus Even in hot weather, can survive for months
Serpulina hyodysenteriae

(Swine Dysentery)

Can survive for several months in manure pits and lagoons, about 1 week in manure at 20oC
Salmonella cholerasuis Months or even years in manure
Erysipelathrix rhusiopathiae Up to 6 months in feces during cool temperatures
Leptospira sp 2 months in dilute urine
Streptococcus suis Several weeks if cool temperature
Worm eggs Many years
PRRSV Heat labile but can survive at 4oC for 1 month


Airborne Spread

Organisms exhaled from the pig in large droplets, as is common with most respiratory bacterial infections, generally travel a short distance, likely less than 5 m. Examples of diseases that can be spread a short distance by aerosol droplets include pleuorpneumonia (A. pleuropneumoniae), Streptococcus suis, Atrophic rhinitis (Pasteurella multocida), and Glasser's disease (H. parasuis).

Certain respiratory pathogens, particularly viruses, can be transmitted several kilometres in wind if conditions are ideal. The best examples of long distance (> 5 km) airborne spread are foot and mouth disease and pseudorabies. It has been suggested that PRRS virus, influenza virus, and Mycoplasma hyopneumoniae can travel up to 1 or 2 kilometres in the air.

The risk analysis of the location of isolation units includes a balance of the chance of disease entry, given the modes of disease transmission listed above, and the costs of building and managing the isolation facility. The isolation unit requires power, water, manure disposal system, and labour. The location of the unit needs to take into consideration wind direction, natural disease barriers such as hills and woods, and road access to the unit. Those constructing the unit need to focus on manure disposal, rodent control, loading facilities for pigs and feed, and shower facilities for employees.

Table 7. Distances between herds that broke with disease and the period over which the closest neighbour was free of the disease, as observed by Muirhead (1989).
Organism/ Disease Distance Time
A. pleuropneumonia 150 m 6 years
Atrophic rhinitis 300 m 3 years
Enzootic pneumonia 150 m 4 months

(Depop of infected herd)

Strep suis 300 m 12 years
Swine Dysentery 300 m 4 years
TGE 400 m 4 months

(disease died out)

Adapted from Muirhead, Pigletter 1989;9(10):37.

People

The role of people in the transmission of pig diseases between farms is over emphasized. The provision of boots and protective clothing to all visitors greatly reduces any risk of disease spread. Whether visitors and employees should be required to shower prior to entering the swine facility is questionable, unless they have recently been in another swine unit. There are cases where veterinarians and farm workers have transmitted foot and mouth disease and TGE from one herd to another. A shower policy and the requirement of pig freedom of 24 to 48 hours do foster an awareness of disease risk and are important if only for this reason. Likewise, a perimeter fence, locked doors, and signs indicating that entry is restricted are important reminders of the importance of biosecurity.

Management techniques used to reduce clinical signs in disease positive herds are aimed at decreasing the volume of disease organisms in the environment and therefore prevent adequate contact. These include all-in all-out, cleaning and disinfecting between batches, and batch farrowing. Segregated early weaning and medicated early weaning are methods to reduce the bacterial and viral load in a group of pigs to prevent adequate contact.

Table 8. Precautions implemented on 122 farms in Britain and the number of herds that broke with disease.
Precaution

Time away from pigs (hrs)

Boots &

Coveralls

None
48 24
Total number of Farms 13 28 50 31
Atrophic rhinitis 0 2 1 1
Enzootic pneumoniaa 9 11 1 0
Strep suis 1 3 2 1
Swine Dysentery 0 0 1 3

a It is assumed that enzootic pneumonia was spread in the air.

Adapted from Muirhead, Pigletter 1989;9(10):38.

References

Muirhead, M.R. (1989) Payoff can justify high health status. Pigletter 9:36-38.